ESPN 54th Annual Meeting

ESPN 2022


 
Using the urine proteomic spectrum to observe children having Chronic Kidney Disease
EKATERINA P. KRIVONOSOVA GADGY M. LETIFOV

1- Federal State Budgetary Educational Institution of Higher Education "Rostov State Medical University" of the Ministry of Health of the Russian Federation
 
Introduction:

The study of the proteomic spectrum of urine allows us to assess the likelihood of progression of various nephropathies, which is relevant, given the increasing incidence of diseases of the urinary system in children, including those with asymptomatic course.

The aim of the study was to search for informative non-invasive markers of renal parenchyma damage in children with chronic kidney disease.

Material and methods:

 Proteomic study of urine was performed using proteomics methods (MALDI-TOF-MS/MS, Ultraflex II, Bruker, USA). Information about molecular interactions was obtained using the STRING 10.0 database. The study included 30 children aged 1 to 18 years with chronic kidney disease, the leading laboratory symptoms were microalbuminuria (MAU) and microhematuria.

Results:

 The level of MAU in the examined children was: A0 (up to 10 mg / day) - in 17% (5 patients), A1 (from 10 to 30 mg / day) - in 13% (4 children), A2 (from 30 to 299 mg / day) - in 60% (18 children), A3 (from 300 mg / day and more) - in 10% (3 people). 45 different proteins have been isolated. In isolated erythrocyturia, tubulointerstitial nephritis antigen (100%), aquaporin-1 (75%), platelet growth factor β (65%), vasorin (50%), and antiepithelial membrane antigen (50%) were most frequently detected. With the addition of MAU, the frequency of detecting a molecule of damage to the kidney tissue (100%), an apoptosis-inducing factor (100%), aquaporin-1 (100%), and neutrophilic gelatinase-bound lipocalin (75%) increased. As the process progressed, in stage III chronic kidney disease, the proteomic spectrum of urine was determined by the factor stimulating prostacyclin, interleukin 16, matrix metalloproteinase, tolloid-like protein 2.

Conclusions:

 Thus, the assessment of the proteomic spectrum of urine makes it possible to identify non-invasive markers of the progression of damage to the tubulointerstitial tissue of the kidneys associated with various pathologies accompanied by MAU and microhematuria.